Increasing the number of ponds running under the same conditions increases the sample size (n) and increases the precision of the measurements (equation 1,2). The second advantage of of having ponds in triplicate is statistical. Having triplicate ponds is important evaluative tool for identifying measurements that fall outside the expected range. This pond can then be carefully evaluated to determine what conditions (if any) are leading to the much higher productivities. For example, if there are three ponds being run in the exact same way and two are demonstrating a productivity of 25 g/m2-day ☒ g/m2-day and the third pond is measuring 85 g/m2-day it is easy to identify the aberrant pond. The first advantage is that triplicate ponds allow clear identification of pond operating outside the expected range. Ponds run in triplicate offer several advantages over ponds run in duplicate. The complexity of this environment introduces significant variability especially when coupled with weather variations such as wind, temperature, precipitation, and solar insolation. Our experience is that triplicates are the optimum given the natural variability in algae cultures.Īlgae raceway ponds are complex biological systems supporting diverse cultures of algae, bacteria, and other organisms. Owning replicate algae raceways saves time and money by decreasing uncertainty in your data. Pond replicates: duplicates, triplicates and robust experimental results
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